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IDEC ABN101

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Description


Key Specifications Table

Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Ca, Po WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number ABN101
Description Anti-GAD65 Antibody
Alternate Names
  • Glutamate decarboxylase 2
  • 65 kDa glutamic acid decarboxylase
  • GAD-65
  • Glutamate decarboxylase 65 kDa isoform
Background Information GAD65 is a group II decarboxylase enzyme that catalyzes the biosynthesis of GABA from glutamate with the aid of the cofactor, pyridoxal 5'-phosphate. GAD65 localizes at presynaptic GABAergic terminals and is reported to supply local vesicular pools of GABA facilitating transient inhibitiory neurotransmission, although GAD65 may also play a role in more sustained tonic inhibitory signals. It is present as a dimer of alpha and beta subunits and is regulated by alternative splicing, which may produce the GAD67 isoform. GAD65 also undergoes post-translational modifications such as palmitoylation of N-terminal cysteine residues and phosphorylation of multiple alpha-subunit serine residues that enable membrane-anchoring and other vesicular activities. GAD65 has been implicated in neurological conditions resulting from failure of inhibitory neurotransmission such as Huntington's disease and epilepsy. In addition to its CNS presence, GAD65 also produces GABA in pancreatic beta cells and dysfunction in this area may contribute to the pathogenesis of insulin-dependent diabetes mellitus.
Product Information
Format Affinity Purified
Control
  • Rat brain membrane tissue lysate
Presentation Purified Rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level MQ100
Applications
Application Anti-GAD Antibody is an antibody against GAD65 for use in Western Blotting. See below for more information about this anti GAD antibody.
Key Applications
  • Western Blotting
Application Notes Western Blot Analysis: 1 µg/mL from a representative lot detected GAD65 on 10 µg of rat brain membrane tissue lysate. Western Blot performed without Tween.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human GAD65.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Canine
  • Pig
Species Reactivity Note Demonstrated to react with Rat, Mouse, and Human. Predicted to react with Canine and Porcine based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GAD2
  • GAD65
Purification Method Affinity Purfied
UniProt Number
Molecular Weight ~59/63 kDa observed. The calculated molecular weight is 65 kDa, however GAD65 has been shown as a ~59/63 doublet in western blots. (Christgau, S., et al. (1991). Journal of Biological Chemistry. 266(31):21257-21264.) An uncharacterized band appears at ~38 kDa in some lysates.
Product Usage Statements
Quality Assurance Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected GAD65 on 10 µg of rat brain membrane tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
We have  52pcs in stock ,
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